Cyanocobalamin USP/EP Vitamins made in china CAS NO.68-19-9

Characteristics

Dark-red crystalline powder or crystals

Solubility

Sparingly soluble in water and ethanol(96%),practically insoluble in acetone.

Identification

Ratio of optical density

A361/A550nm: 3.15~3.40

A361/A278nm: 1.7~1.88

TLC: Conforms

Chemical:Positive

HPLC：the retention time of major peak of the sample solution corresponds to that of the standard solution

Pseudo cyanocobalamin

USP(separator)

Related substances

Total impurities

≤3.0%

Single impurity

7β，8β-lactocyanocobalamin≤1.0%

50-carboxycyanocobalamin≤0.5%

34-methylcyanocobalamin≤2.0%

32-carboxycyanocobalamin≤1.0%

8-epi-cyanocobalamin≤1.0%

Any other unidentified impurity≤0.5%

Loss on drying

≤12.0%

Assay on dried basis

96.0%~102.0%

Residual solvents

Acetone: ≤ 1000ppm

Ethanol: ≤ 1000ppm

Total plate count(CFU/g)

≤ 100

Yeast and Mould(CFU/g)

≤10

Enterobacteriae 

Negative 

Staphylococcus aureus

Negative 

Pseudomonas aeruginosa

Negative 

Endotoxin 

＜0.35EU/µg